Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ALKBH7 Antibody recognizes endogenous levels of total ALKBH7 protein.
Species Reactivity: Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val34 of human ALKBH7 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The AlkB alkylation repair homolog 7 (ALKBH7, ABH7) is a member of the alkylated DNA repair homolog family that is responsible for repair of DNA damage induced by oxidation and alkylation (1). ALKBH7 is a nuclear encoded protein that contains an amino-terminal mitochondrial targeting sequence that directs import of ALKBH7 to the mitochondria (2). Unlike other Alkb family members, the ALKBH7 protein lacks a functional nucleotide recognition lid essential for nucleobase-binding, which abrogates any DNA or RNA repair capability (3). In response to DNA damage, mitochondrial ALKBH7 triggers the collapse of the mitochondrial membrane potential. The resultant loss of mitochondrial function leads to depletion of cellular energy and programmed cell death (2). Research studies indicate that ALKBH7 knockdown cells are resistant to apoptotic cell death induced by oxidizing and alkylating agents, which suggests that ALKBH7 may play a novel function in promoting cell death (2). Indeed, ALKBH7 has been identified as a key regulator of the alkylation or oxidizing DNA damaged induced necroptosis pathway (2).
Alternate Names
ABH7; alkB homolog 7; alkB, alkylation repair homolog 7; alkB, alkylation repair homolog 7 (E. coli); ALKB7; ALKBH7; Alkylated DNA repair protein alkB homolog 7; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial; MGC10974; probable alpha-ketoglutarate-dependent dioxygenase ABH7; SPATA11; spermatogenesis associated 11; spermatogenesis cell proliferation related protein; Spermatogenesis cell proliferation-related protein; Spermatogenesis-associated protein 11; UNQ6002
