Description
Product Usage Information
For optimal ChIP and ChIP-seq results, use 5μl of antibody and 10μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 .
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Immunoprecipitation: 1:200
Immunohistochemistry (Paraffin): 1:50
Immunofluorescence (Frozen): 1:200 - 1:800
Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600
Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400
Chromatin IP: 1:100
Chromatin IP-seq: 1:100
CUT&RUN: 1:100
CUT&Tag: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 97279 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP, Chromatin IP-seq, CUT&RUN, CUT&Tag
Specificity / Sensitivity
Aiolos (D1C1E) Rabbit Monoclonal Antibody recognizes endogenous levels of total Aiolos protein.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro46 of human Aiolos protein.
Background
Aiolos is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family members (1). Multiple Aiolos isoforms are generated through alternative splicing of the portion of the transcript encoding the amino-terminal zinc fingers (2). Aiolos is expressed by lymphoid tissues, with highest expression levels seen in mature B and T cells (1). Ikaros family proteins control lymphocyte development by recruiting chromatin remodeling complexes to DNA (3). B cells from mice lacking Aiolos have a reduced threshold for activation, increased proliferation, and elevated levels of IgG and IgE. In addition, Aiolos mice develop B cell lymphomas (4). In T cells, Aiolos contributes to Th17 cell differentiation by suppressing IL-2 expression (5). Aberrant expression of Aiolos in transformed epithelial cells promotes anchorage independence through downregulation of adhesion-related genes (6). Alterations in the gene are observed in near haploid acute lymphoblastic leukemia, and the genetic locus containing Aiolos is linked to increased susceptibility to rheumatoid arthritis and systemic lupus erythematosus (7-9).
Alternate Names
AIO; AIOLOS; IKAROS family zinc finger 3; IKAROS family zinc finger 3 (Aiolos); Ikaros family zinc finger protein 3; IKZF3; zinc finger DNA binding protein Aiolos; Zinc finger protein Aiolos; zinc finger protein, subfamily 1A, 3 (Aiolos); ZNFN1A3
