Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:3000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
ANP32A (D7Z5U) Rabbit Monoclonal Antibody recognizes endogenous levels of total ANP32A protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino terminus of human ANP32A protein.
Background
ANP32A is the founding member of the acidic nuclear phosphoprotein 32 kDa (ANP32) family, which share two highly conserved regions: the N-terminal leucine-rich repeats (LRRs) sequence and the C-terminal acidic tail (1). ANP32A was originally purified as a potent, heat-stable protein phosphatase 2A (PP2A)-specific inhibitor, and subsequent studies suggested that it binds directly to the catalytic subunit of PP2A (2,3). ANP32A is a key component of acetyltransferase inhibitor complex that regulates chromatin remodeling and transcription (4,5). ANP32A also forms a multiunit complex with HuR that regulates RNA transport and stability (6). In addition, ANP32A is reported to play roles in apoptosis, neural differentiation and pathogenesis (7-10).
Alternate Names
acidic (leucine-rich) nuclear phosphoprotein 32 family, member A; Acidic leucine-rich nuclear phosphoprotein 32 family member A; acidic nuclear phosphoprotein 32 family member A; Acidic nuclear phosphoprotein pp32; AN32A; ANP32A; C15orf1; cerebellar leucine rich acidic nuclear protein; epididymis secretory sperm binding protein; hepatopoietin Cn; HPPCn; I1PP2A; inhibitor-1 of protein phosphatase-2A; LANP; Leucine-rich acidic nuclear protein; MAPM; Mapmodulin; MGC119787; MGC150373; PHAP1; PHAPI; Potent heat-stable protein phosphatase 2A inhibitor I1PP2A; PP32; Putative HLA-DR-associated protein I; putative human HLA class II associated protein I
