Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Arginase-2 Antibody recognizes endogenous levels of total arginase-2 protein. This antibody cross-reacts with arginase-1 protein.
Species Reactivity: Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro338 of human arginase-2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Arginase-2 is a mitochondrial enzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea (1). Research studies have shown that in acute myeloid leukemia (AML) patients, arginase-2 is released from AML blasts to the plasma, leading to the suppression of T-cell proliferation (2). It was also shown that arginase-2 is required for the immunosuppressive properties of neonatal CD71(+) erythroid cells, which inhibits neonatal host defense against infection (3). In addition, the expression of arginase-2 in dendritic cells is repressed by microRNA-155 during maturation (4). This repression is essential for T-cell activation and response (4).
Alternate Names
ARG2; ARGI2; arginase 2; Arginase II; Arginase-2, mitochondrial; arginase, type II; kidney arginase; Kidney-type arginase; L-arginine amidinohydrolase; L-arginine ureahydrolase; Non-hepatic arginase; nonhepatic arginase; Type II arginase
