Description
Product Usage Information
Immunofluorescence (Frozen): 1:200
Immunofluorescence (Immunocytochemistry): 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
AE1/SLC4A1 (D3X1R) Rabbit Monoclonal Antibody (IF Formulated) recognizes endogenous levels of total AE1 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala176 of human AE1 protein.
Background
Anion exchange protein 1 (AE1), also named solute carrier family 4 member 1 (SLC4A1), is an anion transporter that mediates chloride-bicarbonate exchange in the kidney and regulates normal acidification of the urine (1,2). A different isoform of AE1 is a major integral membrane structure protein of erythrocytes, where it plays a critical role in the removal of carbon dioxide from tissues (3). In addition, AE1 is required for normal flexibility and stability of the erythrocyte membrane. Mutations in can lead to hereditary spherocytosis, ovalocytosis, and distal renal tubular-acidosis (4-7). Other mutations that do not cause disease became novel blood group antigens, which are part of the Diego blood group system (8).
Alternate Names
AE 1; AE1; Anion exchange protein 1; Anion exchanger 1; anion exchanger-1; B3AT; band 3 anion exchanger; Band 3 anion transport protein; BND3; CD233; CHC; DI; Diego blood group; EMPB3; EPB3; erythrocyte membrane protein band 3; erythroid anion exchange protein; FR; Froese blood group; MGC116750; MGC116753; MGC126619; MGC126623; RTA1A; SAO; SLC4A1; solute carrier family 4 (anion exchanger), member 1 (Diego blood group); Solute carrier family 4 member 1; solute carrier family 4 member 1 (Diego blood group); solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group); solute carrier family 4, anion exchanger, number 1; SPH4; SW; Swann blood group; Waldner blood group; WD; WD1; WR; Wright blood group
