Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
APOC2 (F4Q3M) Rabbit Monoclonal Antibody recognizes endogenous levels of total APOC2 protein. This antibody detects a 45 kDa and a 65 kDa protein of unknown identity in some cell lines.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val93 of human APOC2 protein.
Background
Apolipoprotein C-II (APOC2) is a secreted cofactor that promotes triglyceride hydrolysis by lipoprotein lipase (LPL) (1). Several pathogenic APOC2 variants that lead to APOC2 deficiency and high triglyceride levels have been identified (2,3). There is also data suggesting that APOC2 is upregulated in various forms of cancer and may influence metabolic pathways of cancer cells (4). In acute myeloid leukemia, APOC2 was found to be highly expressed and to promote cancer cell proliferation by interacting with CD36 and upregulating signaling through Lyn and Erk (5). APOC2 was also found to be overexpressed in gastric cancer and to promote cancer cell invasion, migration, and proliferation by upregulation of PI3K, Akt, and mTOR signaling (6).
Alternate Names
APC2; Apo-CII; ApoC-II; APOC2; Apolipoprotein C-II; Apolipoprotein C2; MGC75082; ProapoC-II; Proapolipoprotein C-II
