Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Abi3 Antibody recognizes endogenous levels of total Abi3 protein. This antibody recognizes a band at ~100 kDa of unknown origin.
Species Reactivity: Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu182 of human Abi3 protein. Antibodies are purified by peptide affinity chromatography.
Background
Abi1, Abi2, and Abi3 are members of the Abl1 interactor family (Abi), which function as adaptor signaling molecules downstream of the receptor tyrosine kinase Abl1 (1-3). In addition to binding to Abl1, all Abi proteins contain a Src homology 3 (SH3) domain and interact with the WAVE complex, an actin regulator involved in membrane ruffling and lamellipodia formation (4,5). As cytoskeletal adaptor proteins, Abi proteins are likely to regulate actin cytoskeletal remodeling. Interestingly, , the gene encoding Abi3, is genetically linked to Alzheimer's disease (AD). Abi3 may contribute to microglia-mediated AD progression by regulating microglial morphology (6,7). Â
Alternate Names
ABI family member 3; ABI family, member 3; ABI gene family member 3; ABI gene family, member 3; ABI3; NESH; New molecule including SH3; SSH3BP3
