Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ACAA2 (F4Y9U) Rabbit Monoclonal Antibody recognizes endogenous levels of total ACAA2 protein. This antibody does not cross-react with ACAA1 protein.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val219 of human ACAA2 protein.
Background
Acetyl-CoA acyltransferase 2 (ACAA2), also known as medium-chain 3-ketoacyl-CoA thiolase, is a mitochondrial enzyme that participates in the catalyzation of the last step in the fatty acid β-oxidation pathway (1,2). Studies identified a short-chain carbon circuit at the C4 node of fatty acid oxidation (FAO) where reverse flux of acetyl-CoA derived from pyruvate through ACAA2 produces free CoA and NAD , therefore promoting lipid tolerance and redox stability in heart mitochondria (3).
Alternate Names
3-ketoacyl-CoA thiolase, mitochondrial; ACAA2; Acetyl-CoA acetyltransferase; Acetyl-CoA acyltransferase; acetyl-CoA acyltransferase 2; acetyl-Coenzyme A acyltransferase 2; Acyl-CoA hydrolase, mitochondrial; beta ketothiolase; Beta-ketothiolase; DSAEC; FLJ35992; FLJ95265; Mitochondrial 3-oxoacyl-CoA thiolase; mitochondrial 3-oxoacyl-Coenzyme A thiolase; T1; THIM
