Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
AGPS (F1N3Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total AGPS protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu120 of human AGPS protein.
Background
Genome-wide CRISPR-Cas9 suppressor screens show that peroxisomes contribute to ferroptosis in human renal and ovarian carcinoma cells by synthesizing polyunsaturated ether phospholipids (PUFA-ePLs). PUFA-ePLs serve as substrates for lipid peroxidation. Genes identified in the screens include those for alkylglycerone phosphate synthase (AGPS) and fatty acyl-CoA reductase 1 (FAR1), two peroxisomal enzymes catalyzing ether lipid biosynthesis. Studies suggest that AGPS and FAR1 contribute to ferroptosis sensitivity (1,2). In addition, research studies show that AGPS suppresses prostate cancer cell proliferation by enhancing ferroptosis (3).
Alternate Names
ADAP-S; ADAS; ADHAPS; ADPS; Aging-associated gene 5 protein; aging-associated protein 5; AGPS; ALDHPSY; Alkyl-DHAP synthase; alkyldihydroxyacetone phosphate synthase; Alkyldihydroxyacetonephosphate synthase, peroxisomal; alkylglycerone phosphate synthase; Alkylglycerone-phosphate synthase; DKFZp762O2215; FLJ99755; RCDP3
