Description
Product Usage Information
Immunofluorescence (Frozen): 1:50
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Immunofluorescence (Frozen)
Specificity / Sensitivity
Bassoon (D63B6) Rabbit Monoclonal Antibody (Alexa Fluor
Species Reactivity: Mouse, Rat
Background
Bassoon (BSN), named such to underline its presumptive role in orchestrating events of the synaptic vesicle cycle (1), is a very large coiled-coil protein and is one of the core components of the cytomatrix at the active zones of both excitatory and inhibitory synapses (2). BSN is a scaffold protein that is a component of the synaptic ribbon, an electron-dense structure anchored at the active zone that tethers synaptic vesicles. Genetic disruption of BSN displaces the anchoring of ribbons to the active zones of photoreceptors and cochlear inner hair cells (3), and this displacement of the ribbons substantially impairs synaptic transmission, suggesting that, when present, BSN is important for the vesicle cycle (4).
Alternate Names
bassoon (presynaptic cytomatrix protein); bassoon presynaptic cytomatrix protein; BSN; KIAA0434; neuronal double zinc finger protein; Protein bassoon; Zinc finger protein 231; ZNF231
