Description
Storage
Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody .
Protocol
Available protocols: Single Cell Analysis - InTraSeq™ 3'
Specificity / Sensitivity
CD8 alpha (SK1) Mouse Monoclonal Antibody (InTraSeq™ 3' Conjugate 3030) recognizes endogenous levels of total CD8α protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with human peripheral blood T cells.
Background
Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen-Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR-antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1).
Alternate Names
CD8; CD8 antigen alpha polypeptide; CD8 antigen, alpha polypeptide (p32); CD8A; CD8a molecule; Leu2; Leu2 T-lymphocyte antigen; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen
