Description
Protocol
Available protocols: ELISA+
Specificity / Sensitivity
The FastScan™ Phospho-SLP-76 (Ser376) ELISA Kit II detects endogenous levels of SLP-76 when phosphorylated at Ser376 as shown in Figure 1. This kit is able to detect phospho-SLP-76 (Ser376) in human samples but is not cross-reactive with mouse phospho-SLP-76 (Ser376), as determined through in-house testing, but may also detect homologous proteins from other species. The FastScan™ Phospho-SLP-76 (Ser376) ELISA Kit II #25424 provides better performance when detecting human phospho-SLP-76 (Ser376) as compared to kit #30794.
Species Reactivity: Human
Background
SH2 domain-containing leukocyte protein of 76 kDa (SLP-76) is a hematopoietic adaptor protein that is important in multiple biochemical signaling pathways and necessary for T cell development and activation (1). ZAP-70 phosphorylates SLP-76 and LAT as a result of TCR ligation. SLP-76 has amino-terminal tyrosine residues followed by a proline-rich domain and a carboxy-terminal SH2 domain. Phosphorylation of Tyr113 and Tyr128 result in recruitment of the GEF Vav and the adaptor protein Nck (2). TCR ligation also leads to phosphorylation of Tyr145, which mediates an association between SLP-76 and Itk, which is accomplished in part via the proline-rich domain of SLP-76 and the SH3 domain of Itk (3). Furthermore, the proline-rich domain of SLP-76 binds to the SH3 domains of Grb2-like adaptor Gads (3,4). In resting cells, SLP-76 is predominantly in the cytosol. Upon TCR ligation, SLP-76 translocates to the plasma membrane and promotes the assembly of a multi-protein signaling complex that includes Vav, Nck, Itk, and PLCγ1 (1). The expression of SLP-76 is tightly regulated; the protein is detected at very early stages of thymocyte development, increases as thymocyte maturation progresses, and is reduced as cells mature to CD4 CD8 double-positive thymocytes (5).
Alternate Names
76 kDa tyrosine phosphoprotein; LCP2; Lymphocyte cytosolic protein 2; lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa); SH2 domain-containing leukocyte protein of 76 kDa; SH2 domain-containing leukocyte protein of 76kD; SLP-76; SLP-76 tyrosine phosphoprotein; SLP76
