Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
AP4B1 Antibody recognizes endogenous levels of total human AP4B1 protein. Weak reactivity has been observed in rodent.
Species Reactivity: Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile708 of human AP4B1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
AP (adaptor protein) complexes regulate intracellular membrane trafficking along endocytic and secretory pathways (1). There are five AP complexes (AP1-5) that exist as heterotetrameric complexes functioning at distinct intracellular compartments. The AP4 complex, which includes AP4E1, AP4B1, AP4M1, and AP4S1, functions at the -Golgi network (TGN)Â (2-4). AP-4 associates with Atg9A which is critical in autophagosome biogenesis (5). Deficiency in AP-4 led to Atg9A retention within the -Golgi network with defective axonal autophagosome generation (5). AP-4 deficiency has been linked to a group of neurodegenerative diseases known as hereditary spastic paraplegia. Mutation in the gene was identified as a cause for the neurological disorder hereditary spastic paraplegia type 47 (SPG47) (6,7).
Alternate Names
Adapter-related protein complex 4 subunit beta-1; adaptor related protein complex 4 beta 1 subunit; adaptor related protein complex 4 subunit beta 1; Adaptor-related protein complex 4 subunit beta-1; adaptor-related protein complex 4, beta 1 subunit; AP-4 adapter complex subunit beta; AP-4 adaptor complex subunit beta; AP-4 complex subunit beta-1; AP4B1; beta 4 subunit of AP-4; Beta subunit of AP-4; BETA-4; Beta4-adaptin; CPSQ5; spastic paraplegia 47; SPG47
