CST, 27398S, Human FGF-acidic/FGF1 Recombinant Protein

Storage
Human FGF-acidic/FGF1 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Source / Purification
Recombinant human FGF-acidic was expressed in and is supplied in a lyophilized form.
Background
FGF-acidic (FGF1) is a potent growth factor for fibroblasts and endothelial cells (1). FGF-acidic is involved in wound repair, angiogenesis, and development (1). FGF-acidic is secreted from cells via an endoplasmic reticulum/Golgi independent mechanism (1,2). The ability of FGF-acidic to bind to heparin sulfate is required for its ability to interact with FGF receptors and induce signaling (1-4). There are four distinct FGF receptors and each has multiple splice variants (1,3). FGF-acidic binds with high affinity to many, but not all, FGFRs (1). Signaling cascades activated through FGF-basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/Akt pathways (1).