Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Adiponectin (C45B10) Rabbit Monoclonal Antibody detects endogenous levels of total adiponectin protein monomer. This antibody will not detect higher molecular weight forms of adiponectin.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human adiponectin protein.
Background
Adiponectin, also termed AdipoQ, Acrp30, apM1 and GBP28, is an adipokine expressed exclusively in brown and white adipocytes (1). It is secreted into the blood and exists in three major forms: a low molecular weight trimer, a medium molecular weight hexamer and a high molecular weight multimer (1). Adiponectin levels are decreased in obese and insulin-resistant mice and humans (2), suggesting that this adipokine is critical to maintain insulin sensitivity. Adiponectin stimulates the phosphorylation of AMPKα at Thr172 and activates AMPK in skeletal muscle (3). It also stimulates glucose uptake in myocytes (3). The block of AMPK activation by a dominant-negative AMPKα2 isoform inhibits the effect of adiponectin on glucose uptake, indicating that adiponectin stimulates glucose uptake and increases insulin sensitivity through its action on AMPK (3). Adiponectin mutants that are not able to form oligomers larger than trimers have no effect on the AMPK pathway (4). Mutations that render adiponectin unable to form high molecular weight multimers are associated with human diabetes (4), indicating the importance of multimerization for adiponectin activity.
Alternate Names
30 kDa adipocyte complement-related protein; ACDC; ACRP30; ADIPO; Adipocyte complement-related 30 kDa protein; Adipocyte, C1q and collagen domain-containing protein; Adiponectin; adiponectin, C1Q and collagen domain containing; ADIPOQ; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; ADIPQTL1; ADPN; apM-1; APM1; GBP28; Gelatin-binding protein; gelatin-binding protein 28
