Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Annexin A6 Antibody recognizes endogenous levels of total annexin A6 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr645 of human annexin A6 protein. Antibodies are purified by peptide affinity chromatography.
Background
Annexin A6 (ANXA6) belongs to the highly conserved annexin family of proteins characterized by their calcium (Ca )-dependent binding to phospholipids (1-3). The protein is highly expressed in most tissues and is predominantly located at the plasma membrane and the endosomal compartment, where it regulates cell migration and endosome trafficking by organizing membrane domains, interacting with signaling complexes, and forming transient membrane-actin interactions upon Ca stimulation (3). It also mediates intracellular cholesterol homeostasis (3,4). Annexin A6 is frequently upregulated in extracellular vesicles (EVs) of the tumor microenvironment after chemotherapy or tyrosine kinase inhibitor treatment. These EVs have been shown to promote resistance and metastasis in multiple cancer types, such as lung cancer, breast cancer, and pancreatic duct adenocarcinoma (5-7). Annexin A6, in these EVs, enhances the stability of membrane-associated RTKs, such as EGFR, and promotes cancer cell motility, stemness, and autophagy (2,7-9).
Alternate Names
67 kDa calelectrin; Annexin A6; Annexin VI; annexin VI (p68); Annexin-6; ANX6; anxa 6; ANXA6; calcium-binding protein p68; calelectrin; calphobindin II; Calphobindin-II; CBP68; Chromobindin-20; CPB-II; Lipocortin VI; p68; p70; Protein III; testis secretory sperm-binding protein Li 198a
