Description
Product Usage Information
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Immunoprecipitation
Specificity / Sensitivity
Akt (5G3) Mouse Monoclonal Antibody detects endogenous levels of Akt1 and Akt3. This antibody does not cross-react with other related proteins.
Species Reactivity: Human, Mouse, Rat, Hamster
Source / Purification
Monoclonal antibody is produced by immunizing animals with an Akt1 recombinant protein containing human Akt1 residues 140-480.
Background
Akt, also referred to as PKB or Rac, plays a critical role in controlling cell survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3K/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin-dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).
Alternate Names
AKT; AKT serine/threonine kinase 1; AKT serine/threonine kinase 3; AKT1; AKT1 kinase; AKT1m; AKT3; DKFZp434N0250; MGC99656; MPPH; MPPH2; PKB; PKB alpha; PKB gamma; PKB-ALPHA; PKB-GAMMA; PKBG; PRKBA; PRKBG; Protein kinase Akt-3; Protein kinase B; Protein kinase B alpha; Protein kinase B gamma; Proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-gamma; RAC-gamma serine/threonine protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-gamma; serine threonine protein kinase, Akt-3; serine-threonine protein kinase; STK-2; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); v-akt murine thymoma viral oncogene-like protein 1
