Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:50 - 1:250
Immunoprecipitation: 1:100
Immunohistochemistry (Paraffin): 1:250 - 1:1000
Immunofluorescence (Frozen): 1:800 - 1:1600
Immunofluorescence (Immunocytochemistry): 1:200 - 1:400
Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
Angiomotin (F7W8U) Rabbit Monoclonal Antibody recognizes endogenous levels of total angiomotin protein. Based on the sequence of the immunogenic peptide, this antibody is not expected to cross-react with the AMOT family members AMOTL1 or AMOTL2.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1075 of human angiomotin protein.
Background
Angiomotin (AMOT) is a widely expressed cell junction protein initially identified through its ability to bind angiostatin. Alternative splicing results in two isoforms of angiomotin, the full-length p130 and the amino-terminally truncated p80. These isoforms, along with angiomotin-like 1 (AMOTL1) and angiomotin-like 2 (AMOTL2), comprise the Motin protein family. Angiomotin is important in endothelial cell polarity, migration, and blood vessel formation during development, as well as in signaling through small GTPases and the Hippo/YAP pathway (reviewed in 1). Research studies have shown that angiomotin expression regulates migration and proliferation of breast cancer cells (2,3).
Alternate Names
AMOT; Angiomotin; angiomotin p130 isoform; angiomotin p80 isoform; KIAA1071
