Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Immunohistochemistry (Paraffin): 1:1000 - 1:4000
Immunofluorescence (Immunocytochemistry): 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
ALPP/ALPPL2 (F1L4G) Rabbit Monoclonal Antibody recognizes endogenous levels of total ALPP and ALPPL2 protein. This antibody does not cross-react with ALPI or ALPL protein but it may cross-react with a protein of unknown origin at 25 kDa.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human ALPP protein.
Background
Mammalian alkaline phosphatases (ALPs) are highly conserved zinc-containing allosteric enzymes that hydrolyze and transphosphorylate a wide range of compounds (1). There are four known human alkaline phosphatase isozymes: ALPL (tissue-nonspecific; bone/liver/kidney), ALPP (placental), ALPPL2 (germ cell), and ALPI (intestinal) (2). Placental alkaline phosphatase (ALPP) is bound to the plasma membrane via a glycosyl-phosphatidylinositol (GPI) anchor (3). It is expressed primarily in the placenta (4) and may be involved in transplacental IgG transport (5). ALPP has been found to be overexpressed on the surface of several different types of solid tumor cells (6), and elevated serum concentrations of ALPP and ALPP-like enzymes has been found to be associated with ovarian, cervical, and testicular cancer (7).
Alternate Names
Alkaline phosphatase Regan isozyme; alkaline phosphatase, placental; alkaline phosphatase, placental (Regan isozyme); Alkaline phosphatase, placental type; alkaline phosphomonoesterase; ALP; ALPI; ALPP; FLJ61142; glycerophosphatase; IAP; Intestinal alkaline phosphatase; Intestinal-type alkaline phosphatase; PALP; Placental alkaline phosphatase 1; PLAP; PLAP-1; PPB1
