CST, 3208S, AP-2 alpha Antibody

Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
AP-2 alpha Antibody detects endogenous levels of total AP-2α protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence of human AP-2α. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The sequence-specific transcription factor activator protein 2α (AP-2α) is required for normal growth and morphogenesis during mammalian development (1,2). Decreased or loss of AP-2α expression has been observed in many different types of human cancers including breast cancer (3,4), ovarian cancer (5), melanoma (6) and prostate cancer (7). These findings suggest that AP-2α expression plays a crucial role in tumorigenicity. Studies have also shown that p53 overexpression in human breast carcinoma cells induces the level of AP-2α expression. Furthermore, p53 binds to the -element in the AP-2α promoter, suggesting that AP-2α is a target of p53. AP-2α may mediate the effect of p53 to inhibit cell proliferation (8).
Alternate Names
Activating enhancer-binding protein 2-alpha; Activator protein 2; AP-2; AP-2 alpha; AP-2 transcription factor; AP-2alpha; AP2-alpha; AP2A; AP2TF; BOFS; FLJ51761; TFAP2; TFAP2A; transcription factor AP-2 alpha; transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha); Transcription factor AP-2-alpha