CST, 32774S, Aphidicolin

Product Usage Information
Aphidicolin is supplied as a lyophilized powder. For a 5 mM stock, reconstitute 1 mg of powder in 590 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Storage
Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Background
Aphidicolin is a tetracyclic diterpenoid first produced by the fungus . This antibiotic is a potent inhibitor of cellular deoxyribonucleic acid (DNA) synthesis by targeting α, ε, and δ DNA polymerases in eukaryotic cells lines with little to no effect on β or γ DNA polymerases (1,2). Studies have shown that Aphidicolin specifically binds to α DNA polymerase, resulting in the formation of a pol α-DNA-aphidicolin ternary complex that blocks DNA replication. The uses for this compound include synchronized cells at the G1/S boundary and increased gene amplification (3-6).
Alternate Names
(+)-Aphidicolin; (3R,4R,4aR,6aS,8R,9R,11aS,11bS)-Tetradecahydro-3,9-dihydroxy-4,11b-dimethyl-8,11a- methano-11aH-cyclohepta[a]naphthalene-4,9-dimethanol; (from Nigrospora sp.); ICI 69653; Nigrospora sp.; NSC 234714