Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
AP1S1 (F8K5S) Rabbit Monoclonal Antibody recognizes endogenous levels of total AP1S1 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AP1S1 protein.
Background
The adaptor protein complex 1 (AP-1) is a heterotetrameric protein complex composed of two large adaptins (AP1G1 and AP1B1), a medium adaptin (AP1M1 or AP1M2), and one of several small adaptins (AP3S1, AP1S2, or AP1S3). AP-1 complexes containing AP1M1 are ubiquitously expressed, while complexes containing AP1M2 display a more restricted tissue expression pattern. Research studies have demonstrated that the AP-1 complex is localized to the trans-Golgi network and recycling endosomes where it regulates the bidirectional trafficking of cargo proteins between these subcellular compartments as well as the biogenesis of secretory vesicles (1-3). AP1S1 is one of three possible small adaptin subunits capable of being incorporated into the AP-1 heterotetrameric complex. Mutations in contribute to MEDNIK syndrome, which is characterized by a defect in copper metabolism due to aberrant sorting and trafficking of copper pumps ATP7A and ATP7B (4).
Alternate Names
Adapter-related protein complex 1 sigma-1A subunit; Adaptor protein complex AP-1 sigma-1A subunit; Adaptor protein complex AP-1 subunit sigma-1A; adaptor related protein complex 1 sigma 1 subunit; adaptor related protein complex 1 subunit sigma 1; Adaptor-related protein complex 1 subunit sigma-1A; adaptor-related protein complex 1, sigma 1 subunit; AP-1 complex subunit sigma-1A; AP19; AP1S1; CLAPS1; Clathrin assembly protein complex 1 sigma-1A small chain; Clathrin coat assembly protein AP19; clathrin-associated/assembly/adaptor protein, small 1 (19kD); EKV3; FLJ92436; Golgi adaptor HA1/AP1 adaptin sigma-1A subunit; HA1 19 kDa subunit; MEDNIK; Sigma 1a subunit of AP-1 clathrin; Sigma-adaptin 1A; SIGMA1A; sigma1A subunit of AP-1 clathrin adaptor complex; Sigma1A-adaptin; WUGSC:H_DJ0747G18.2
