CST, 3400S, Myc-Tag (9B11) Mouse Monoclonal Antibody (Sepharose ® Bead Conjugate)

Product Usage Information
Immunoprecipitation: 1:20
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at -20°C. Do not aliquot the antibodies.
Protocol
Available protocols: Immunoprecipitation
Specificity / Sensitivity
Myc-Tag (9B11) Mouse Monoclonal Antibody (Sepharose
Species Reactivity: All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).