Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:50 - 1:250
Immunofluorescence (Frozen): 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Frozen)
Specificity / Sensitivity
ALDH1L1 (E7D7G) Rabbit Monoclonal Antibody recognizes endogenous levels of total ALDH1L1 protein. This product detects only Rodent ALDH1L1 for the IF-F and IF-IC applications.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp158 of human ALDH1L1 protein.
Background
Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), also known as 10-formyltetrahydrofolate dehydrogenase (FDH), is a member of the aldehyde dehydrogenase super family. It is a key enzyme in folate metabolism, playing an important role in regulation of cellular metabolism including proliferation (1,2). Downregulation of ALDH1L1 has been seen in tumors, leading to decreased suppression of cancer cell proliferation (2,3). ALDH1L1 has also been shown to be a useful astrocyte marker throughout the gray and white matter of the brain, labeling both the cell body and processes of astrocytes. ALDH1L1 does not label oligodendrocytes or neurons (4). It has been shown that expression of ALDH1L1 in astrocytes is stably expressed through normal tissue and during astrogliosis (5).
Alternate Names
10-formyltetrahydrofolate dehydrogenase; 10-fTHF; 10-FTHFDH; AL1L1; aldehyde dehydrogenase 1 family member L1; aldehyde dehydrogenase 1 family, member L1; aldehyde dehydrogenase 1L1; Aldehyde dehydrogenase family 1 member L1; ALDH1L1; Cytosolic 10-formyltetrahydrofolate dehydrogenase; DKFZp781N0997; FDH; formyltetrahydrofolate dehydrogenase; FTHFD; MER57B-ALDH1L1
