Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 55569 .
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
alpha-Amylase (D55H10) Rabbit Monoclonal Antibody detects endogenous levels of total α-amylase protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys288 of human α-amylase.
Background
α-amylase catalyzes the cleavage of 1, 4-α-D-glucosidic bonds in oligosaccharides and polysaccharides (1). The enzyme is normally produced and secreted in salivary glands (salivary α-amylase or AMY1) and pancreas (pancreatic α-amylase or AMY2A) (1). Studies reported the release of an ectopically expressed α-amylase in certain tumors (1). Furthermore, a new type of α-amylase (carcinoid α-amylase or AMY2B) was identified in a lung carcinoid tissue (2-4). The ectopic expression of α-amylase in a neuroendocrine tumor was also reported (5).
Alternate Names
1,4-alpha-D-glucan glucanohydrolase 2B; Alpha-amylase 2B; alpha-amylase carcinoid; AMY2; AMY2B; AMY3; amylase alpha 2B; amylase alpha 2B (pancreatic); amylase, alpha 2B (pancreatic); Carcinoid alpha-amylase; glycogenase; HXA
