Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
AMPA Receptor 4 (GluA 4) (Ala60) Antibody detects endogenous levels of total GluA 4 protein.
Species Reactivity: Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala60 of human GluA 4. Antibodies are purified by protein A and peptide affinity chromatography.
Background
AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainate-, and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluA 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the central nervous system. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). In contrast to GluA 2-containing AMPARs, AMPARs that lack GluA 2 are permeable to calcium (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Research studies have implicated activity changes in AMPARs in a variety of diseases including Alzheimer's, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1). GluR 4 containing AMPA receptors are found in synapses and GluR 4 delivery to synapses and cell surface expression is mediated through phosphorylation of Ser842 by PKA or PKC (3).
Alternate Names
AMPA-selective glutamate receptor 4; GluA4; GluR-4; GluR-D; GLUR4; GLUR4C; GLURD; glutamate ionotropic receptor AMPA type subunit 4; Glutamate receptor 4; Glutamate receptor ionotropic, AMPA 4; glutamate receptor, ionotrophic, AMPA 4; glutamate receptor, ionotropic, AMPA 4; GRIA4; NEDSGA
