Description
Product Usage Information
IHC Leica Bond: 1:50 - 1:200
Immunohistochemistry (Paraffin): 1:100 - 1:400
Immunofluorescence (Immunocytochemistry): 1:50 - 1:200
Storage
Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 12 months when stored at 4°C. Do not aliquot the antibody. Note: This product is only available in the format listed above. We are unable to provide this in a carrier free, custom formulation.
Protocol
Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
AFP (C3) Mouse Monoclonal Antibody recognizes endogenous levels of alpha-fetoprotein (AFP).
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with serum of a hepatoma patient that has affinity isolated human alpha-fetoprotein (AFP).
Background
Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Research studies have shown that elevated serum AFP levels are predictive of hepatocellular carcinoma (3).
Alternate Names
AFP; AFPD; alpha fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; Alpha-fetoprotein; FETA; HPAFP
