Description
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml BSA, and 50% glycerol. Store at -20°C.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Streptavidin has a remarkably high affinity for its natural ligand, biotin. The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4).
Species Reactivity: All Species Expected
Source / Purification
Streptavidin-HRP is purified by gel filtration. The conjugate product is free from unconjugated streptavidin and HRP.
Background
Streptavidin is a 53 kDa homotetramer isolated from for use in isolation and detection bioassays (3). Each streptavidin subunit forms high affinity non-covalent bonds with the vitamin biotin. Because of its strong non-covalent interaction with biotin, streptavidin can be used to detect and isolate biotinylated proteins (1,2). Chemiluminescent detection systems have emerged as the best all-around detection method for use with western blots and ELISA. These detection assays eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Streptavidin-HRP is used with biotinylated proteins and specific chemiluminescent substrates to generate light signal. Streptavidin-HRP conjugates have a very high turnover rate, coupling high sensitivity with short reaction times.
Alternate Names
Avidin; SA; secondary; Streptavidin
