Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:500 - 1:2000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
ACBP/DBI (E4V8V) Rabbit Monoclonal Antibody recognizes endogenous levels of total ACBP/DBI protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant human ACBP/DBI protein.
Background
Acyl-CoA-binding protein (ACBP) binds to long-chain acyl-CoA molecules and regulates lipid metabolism. This protein is also known as diazepam-binding inhibitor (DBI) and binds to the benzodiazepine-binding site of the γ-aminobutyric acid (GABA) type A receptor (GABA R), regulating its activity (1). ACBP/DBI is highly expressed in glioblastoma and promotes glioblastoma growth by providing long-chain acyl-CoA molecules to mitochondria for fatty acid oxidation. Reduced ACBP/DBI expression leads to slower proliferation of glioblastoma (2,3). In addition, ACBP/DBI is a regulator of neural progenitor and stem cell proliferation in adult mouse neurogenic niches (4,5). Studies show that ACBP/DBI modulates embryonic neurogenesis of excitatory and inhibitory neurons through GABA signaling (6).
Alternate Names
ACBD1; ACBP; acyl coenzyme A binding protein; Acyl-CoA-binding protein; acyl-Coenzyme A binding domain containing 1; CCK-RP; cholecystokinin-releasing peptide, trypsin-sensitive; DBI; diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein); diazepam binding inhibitor (GABA receptor modulator, acyl-Coenzyme A binding protein); diazepam binding inhibitor, acyl-CoA binding protein; Diazepam-binding inhibitor; Endozepine; EP; epididymis secretory sperm binding protein; GABA receptor modulator; MGC70414
