CST, 4084L, DRAQ5 ®

Product Usage Information
Please follow CST's recommended IF and Flow protocols. For both applications, following secondary detection: Flow Cytometry: Centrifuge cells and resuspend in 0.5 ml DRAQ5 ® diluted 1:500 (10 µM) in PBS or Antibody Dilution Buffer. Incubate for 5 min at room temperature in the dark before analyzing cells on flow cytometer. Immunofluorescence: Rinse samples twice in PBS for five minutes each. Dilute DRAQ5 ® 1:1000 (5 µM) in PBS and incubate for 5 minutes at room temperature in the dark. Rinse samples once in PBS, coat coverslips with anti-fade reagent and examine immediately using appropriate excitation wavelength.
Storage
Store at 4°C protected from light.
Background
DRAQ5 , 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in live or fixed cells. This dye can be used in combination with GFP or FITC labels. DRAQ5 has been used to examine cellular DNA in flow cytometry and fluorescent microscopy applications (1-3).
Alternate Names
cellular dyes; DNA dye; draq