Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Acetyl-CoA Carboxylase 1 Antibody detects endogenous levels of total acetyl-CoA carboxylase 1 protein and does not cross-react with acetyl-CoA carboxylase 2. Immunofluorescence data indicate that the antibody is more reactive to rodent than human proteins.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human acetyl-CoA carboxylase 1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).
Alternate Names
ACAC; ACACA; ACACAD; ACC; ACC-alpha; ACC1; ACCA; Acetyl-CoA carboxylase 1; acetyl-CoA carboxylase alpha; acetyl-CoA carboxylase-alpha; acetyl-Coenzyme A carboxylase alpha; Biotin carboxylase
