CST, 42193S, Cell Fractionation Kit (Eco-friendly detergent)

Storage
Store at -20ºC. Upon receipt, Protease Inhibitor Cocktail (100X) #5871 should be stored at 4ºC. All other components should be stored at -20ºC.
Protocol
Available protocols: Western Blotting, Functional
Background
Cellular fractionation allows for the extraction of cellular proteins into distinct compartments. This is achieved by the use of detergents that take advantage of the inherent qualities and composition of different cellular membranes (1). Cellular fractionation has been important for defining the localization of many proteins, observing the translocation of proteins, and determining protein-protein complexes, such as cytoskeletal-associated proteins (2,3). Thus, detergent-based cellular fractionation separates cellular components with greater ease and speed compared to a more laborious density centrifugation method (4).
Alternate Names
Cellular; cytoplasmic; cytoskeletal; Fractionation; membrane; nuclear; organelle; organelle marker; subcellular; triton free