CST, 42953S, Human Amphiregulin Recombinant Protein

Storage
Human Amphiregulin Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Source / Purification
Recombinant human Amphiregulin was expressed in and is supplied in a lyophilized form.
Background
Amphiregulin (AREG) is essential for mammary ductal development and is expressed in many parts of the body, such as the ovary, placenta, pancreas, breast, lung, and spleen (1,2). Studies on preneoplastic human breast tissue and breast cancer cell lines suggest a significant role for amphiregulin in driving human breast cancer progression (1). Amphiregulin interacts with the epidermal growth factor (EGF) receptor to promote the growth of normal epithelial cells (3). It drives the proliferation of fibroblasts and production of cytokines interleukin 8 (IL-8) and vascular endothelial growth factor (VEGF) which suggests a proinflammatory role as observed in rheumatoid arthritis patients (4).