Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:500 - 1:2000
Immunofluorescence (Immunocytochemistry): 1:200 - 1:800
Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 91519 .
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
AML1 (D33G6) Rabbit Monoclonal Antibody detects endogenous levels of total AML1 protein.
Species Reactivity: Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala36 of human AML1 protein.
Background
AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML, and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8).
Alternate Names
Acute myeloid leukemia 1 protein; AML1; aml1 oncogene; AML1-EVI-1; AML1-EVI-1 fusion partner; AML1-EVI-1 fusion protein; AMLCR1; CBF-alpha-2; CBF2alpha; CBFA2; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; EVI-1; mutant RUNX1; Oncogene AML-1; PEA2-alpha B; PEBP2-alpha B; PEBP2aB; PEBP2alpha; Polyomavirus enhancer-binding protein 2 alpha B subunit; runt related transcription factor 1; Runt-related transcription factor 1; RUNX family transcription factor 1; RUNX1; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit
