Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
APOBEC3G (D9C6Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total APOBEC3G protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly255 of human APOBEC3G protein.
Background
APOBEC3G, a single-strand DNA deaminase that converts deoxycytidine to deoxyuridine (1-3), is a cellular restriction factor blocking the replication of retroviruses (3). It suppresses the replication of human immunodeficiency virus-1 (HIV-1) in T-cells by being packaged into progeny virions and deaminating cytosine to uracil in the nascent minus-strand viral DNA, leading to the accumulation of mutations in the viral genome (1-4). The HIV-1 virion infectivity factor (Vif) inhibits APOBEC3G activity by preventing its incorporation into progeny virions (4,5). In addition, Vif targets APOBEC3G for ubiquitination and subsequent degradation (5).
Alternate Names
A3G; ABC3G; APOBEC-related cytidine deaminase; APOBEC-related protein; APOBEC-related protein 9; APOBEC3G; apolipoprotein B editing enzyme catalytic polypeptide-like 3G; apolipoprotein B mRNA editing enzyme catalytic subunit 3G; apolipoprotein B mRNA editing enzyme cytidine deaminase; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G; apolipoprotein B mRNA-editing enzyme catalytic polypeptide 3G; ARCD; ARP-9; ARP9; bK150C2.7; CEM-15; CEM15; Deoxycytidine deaminase; dJ494G10.1; DNA dC->dU editing enzyme; DNA dC->dU-editing enzyme APOBEC-3G; FLJ12740; MDS019; phorbolin-like protein MDS019
