CST, 4418S, Anti-rat IgG (H+L), (Alexa Fluor ® 647 Conjugate)

Product Usage Information
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:500 - 1:2000 should yield acceptable results for immunofluorescent and flow cytometry assays.
Storage
Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity / Sensitivity
IgG is prepared from goat antibodies that have been adsorbed against mouse IgG, mouse serum and human serum. Cross-reactivity with mouse primary antibodies has been observed.
Species Reactivity: Rat
Background
This product has been optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology's strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.