Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
AP1G1 (F2S8B) Rabbit Monoclonal Antibody recognizes endogenous levels of total AP1G1 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AP1G1 protein.
Background
The adaptor protein complex 1 (AP-1) is a heterotetrameric protein complex composed of two large adaptins (AP1G1 and AP1B1), a medium adaptin (AP1M1 or AP1M2), and one of several small adaptins (AP3S1, AP1S2, or AP1S3). AP-1 complexes containing AP1M1 are ubiquitously expressed, while complexes containing AP1M2 display a more restricted tissue expression pattern. Research studies have demonstrated that the AP-1 complex is localized to the trans-Golgi network and recycling endosomes where it regulates the bidirectional trafficking of cargo proteins between these subcellular compartments as well as biogenesis of secretory vesicles (1-3). The AP-1 complex recognizes dileucine-based sorting motifs within the cytoplasmic tail of cargo proteins, in part, via the AP1G1 subunit (4). In mice, biallelic knockout of is embryonic lethal due to disruption of AP-1 complex stability, highlighting the importance of this subunit to the structure and function of AP-1 (5).
Alternate Names
Adapter-related protein complex 1 subunit gamma-1; Adaptor protein complex AP-1 subunit gamma-1; adaptor related protein complex 1 gamma 1 subunit; adaptor related protein complex 1 subunit gamma 1; Adaptor-related protein complex 1 subunit gamma-1; adaptor-related protein complex 1, gamma 1 subunit; ADTG; AP-1 complex subunit gamma-1; AP1G1; CLAPG1; clathrin assembly protein complex 1 gamma large chain; Clathrin assembly protein complex 1 gamma-1 large chain; clathrin-associated/assembly/adaptor protein, large, gamma 1; gamma adaptin; Gamma1-adaptin; golgi adaptor HA1/AP1 adaptin gamma subunit; Golgi adaptor HA1/AP1 adaptin subunit gamma-1; MGC18255; testicular tissue protein Li 21
