Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ABCG2 Antibody detects endogenous levels of total ABCG2 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr180 of human ABCG2 protein, which lies within the intracellular region of ABCG2. Antibodies were purified by protein A and peptide affinity chromatography.
Background
ABCG2 (BCRP1/ABCP/MXR) is a member of the ATP-binding cassette transporter family that functions as ATP-dependent transporters for a wide variety of chemical compounds and are associated with drug-resistance in cancer cells (1-6). ABCG2 is a heavily glycosylated transmembrane protein with six transmembrane spanning regions consistent with it functioning as a half-transporter. The ABC family can exist as either full-length transporters or as half-transporters that form functional transporters through homo- or heterodimerization. High expression of ABCG2 was found in placenta as well as cell lines selected for resistance to a number of chemotherapeutic drugs, including mitoxantrone, doxorubicin, topotecan and flavopiridol. In rodents, the highest expression of ABCG2 was found in kidney (8). ABCG2 expression has also been observed in stem cell populations, particularly in hematopoietic and neuronal stem cells and is downregulated with differentiation (9-11).
Alternate Names
ABC transporter; ABC15; ABCG2; ABCP; ATP binding cassette subfamily G member 2 (Junior blood group); ATP-binding cassette sub-family G member 2; ATP-binding cassette transporter G2; ATP-binding cassette, sub-family G (WHITE), member 2; ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group); BCRP; BCRP1; BMDP; Breast cancer resistance protein; Broad substrate specificity ATP-binding cassette transporter ABCG2; CD338; CDw338; EST157481; GOUT1; MGC102821; mitoxantrone resistance protein; Mitoxantrone resistance-associated protein; MRX; multi drug resistance efflux transport ATP-binding cassette sub-family G (WHITE) member 2; MXR; MXR-1; MXR1; placenta specific MDR protein; Placenta-specific ATP-binding cassette transporter; UAQTL1; Urate exporter
