Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Immunohistochemistry (Paraffin): 1:1600 - 1:6400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
ALPPL2 (F9B1D) Rabbit Monoclonal Antibody recognizes endogenous levels of total ALPPL2 protein. This antibody does not cross-react with other ALP proteins. This antibody detects a 140 kDa protein of unknown identity in some cell lines.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala89 of human ALPPL2 protein.
Background
Mammalian alkaline phosphatases (ALPs) are highly conserved zinc-containing allosteric enzymes that hydrolyze and transphosphorylate a wide range of compounds (1). There are four known human alkaline phosphatase isozymes: ALPL (tissue-nonspecific; bone/liver/kidney), ALPP (placental), ALPPL2 (germ cell), and ALPI (intestinal) (2). Alkaline phosphatase placental-like 2 (ALPPL2) shares high (98%) homology with alkaline phosphatase placental (ALPP), but is uniquely expressed in the thymus, testis, and certain germ cell cancers such as seminoma (3-6). ALPPL2 has become an attractive immunotherapy target due to its restricted expression in healthy tissue and overexpression in various cancers (7). In addition to seminoma, elevated ALPPL2 expression has been identified in gastric and pancreatic cancers and mesothelioma (7-9).
Alternate Names
Alkaline phosphatase Nagao isozyme; alkaline phosphatase, germ cell; Alkaline phosphatase, germ cell type; alkaline phosphatase, placental like 2; Alkaline phosphatase, placental-like; alkaline phosphatase, placental-like 2; ALP-1; ALPG; ALPPL; ALPPL2; GCAP; Germ cell alkaline phosphatase; Nagao isozyme; Placental alkaline phosphatase-like; placental-like alkaline phosphatase; PLAP-like; PPBN; testicular and thymus alkaline phosphatase
