CST, 45943P, Peptide Desalting C18 StageTip
Miscellaneous for studying in the research area.
Catalog Number: 45943P
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Answer:The pack contains 10 individual C18 StageTips for 10 sample-preparation assays.
Answer:It retains hydrophobic peptides during reversed-phase cleanup so salts and other polar components can be removed before LC-MS.
Answer:Store the StageTips at room temperature. The product has a stated stability of at least 36 months under recommended conditions.
Cell Signaling Technology Peptide Desalting C18 StageTips provide small-scale reversed-phase solid-phase extraction for preparing peptide mixtures before liquid chromatography–mass spectrometry. Hydrophobic peptides bind to the C18 phase while many salts and other highly polar sample components pass through or are removed during washing. Peptides are then recovered with a suitable organic-containing elution solvent for downstream analysis. The compact StageTip format is particularly useful when sample volumes are limited and avoiding unnecessary transfers is important. Typical applications include cleanup of enzymatic digests and peptides released from affinity-enrichment workflows such as PTMScan. Each pack contains ten individual tips for ten assays. Condition the sorbent, load the peptide sample, wash and elute using an established peptide-desalting protocol; recovery depends on peptide properties and solvent conditions. Keep the tips at room temperature under the recommended storage conditions. These consumables are intended for research sample preparation and do not replace analytical separation columns or mass spectrometry instrumentation.
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