CST, 46232S, RBC Lysis Buffer (10X)

Product Usage Information
Note: It is recommended to dilute the RBC Lysis Buffer (10X) just prior to use. 1. Dilute the RBC Lysis Buffer (10X) to a 1X working concentration by adding 1 part RBC Lysis Buffer (10X) to 9 parts room temperature deionized water. Warm the solution to room temperature. 2. Add 2.0 ml of 1X RBC Lysis Buffer to the prepared sample of whole blood (50-100 µL per tube), gently vortex the sample. 3. Incubate for 10-15 minutes at room temperature protected from light. 4. Centrifuge cells at 500 x g for 5 minutes at room temperature. Carefully aspirate the supernatant without disturbing the cell pellet. 5. Wash the cells once in excess with Flow Cytometry Antibody Dilution Buffer # 13616 , repeat the centrifugation in step 4. 6. Resuspend the cells in the appropriate volume for analysis. Note: Above outlines the necessary steps for lysis of human whole blood samples. If working with mouse blood or splenocytes, decrease the incubation time (step 3) to 5 minutes. Further optimization may be required.
Storage
The 10X solution is stable for 6 months when stored at 4°C.