Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Immunohistochemistry (Paraffin): 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
AHCY/SAHH (F2O1K) Rabbit Monoclonal Antibody recognizes endogenous levels of total AHCY/SAHH protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AHCY/SAHH protein.
Background
Adenosylhomocysteinase (AHCY), also known as S-adenosyl-L-homocysteine hydrolase (SAHH), catalyzes the hydrolysis of SAH to generate homocysteine and adenosine (1,2). SAH is a by-product in the transmethylation reactions catalyzed by S-adenosylmethionine (SAM)-dependent methyltransferases. These methylation reactions are critical to various biological processes. SAH is also a feedback inhibitor of SAM-dependent methyltransferases. By catalyzing the above reaction, AHCY/SAHH plays a crucial role in relieving the inhibition (3). Moreover, studies show that hydrogen sulfide (H S) persulfidates Cys195 on AHCY/SAHH in human non-small cell lung cancer (NSCLC) cells and therefore decreases its enzymatic activity, resulting in reduced homocysteine levels. This, in turn, lowers cysteine and glutathione levels in the cell when cystine is depleted, making NSCLC cells more susceptible to ferroptosis (2,4).
Alternate Names
Adenosylhomocysteinase; AdoHcyase; AHCY; epididymis secretory sperm binding protein; S-adenosyl-L-homocysteine hydrolase; S-adenosylhomocysteine hydrolase; SAHH
