Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:200
Immunofluorescence (Immunocytochemistry): 1:100
Flow Cytometry (Fixed/Permeabilized): 1:400
Flow Cytometry (Live): 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 81977 .
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live)
Specificity / Sensitivity
4F2hc/SLC3A2 (D3F9D) Rabbit Monoclonal Antibody recognizes endogenous levels of total 4F2hc/SLC3A2 protein. This antibody is predicted to detect multiple isoforms of 4F2hc/SLC3A2.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val509 of human 4F2hc/SLC3A2 protein.
Background
4F2hc is a transmembrane protein that belongs to the solute carrier family. 4F2hc forms heterodimeric complexes with various amino acid transporters such as LAT1 and LAT2 and regulates uptake of amino acids (1-5). 4F2hc is one of the earliest expressed antigens on the surface of activated human lymphocytes (6), hence it is also named CD98. 4F2hc is expressed in all cell types with the exception of platelets, and is expressed at highest levels in the tubules of the kidney and the gastrointestinal tract (7,8). It is localized at the plasma membrane when associated with LAT1 or LAT2 (9) and at the apical membrane of placenta (10). Research studies have shown that 4F2hc is highly expressed in various tumors including glioma (11), ovarian cancer (12), and astrocytomas (13), and it has been implicated in tumor progression and correlated with poor outcome in patients with pulmonary neuroendocrine tumors (14). 4F2hc is also involved in integrin trafficking through association with β1 and β4 integrins, and regulates keratinocyte adhesion and differentiation (15).
Alternate Names
4F2; 4F2 cell-surface antigen heavy chain; 4F2 heavy chain antigen; 4F2hc; 4T2HC; Amino acid transporter heavy chain SLC3A2; antigen defined by monoclonal antibody 4F2, heavy chain; antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43; CD98; CD98 heavy chain; CD98HC; heavy chain; Lymphocyte activation antigen 4F2 large subunit; MDU1; monoclonal antibody 44D7; NACAE; SLC3A2; solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2; solute carrier family 3 (amino acid transporter heavy chain), member 2; Solute carrier family 3 member 2; solute carrier family 3, member 2
