Description
Storage
Store all components at -20ºC. This product is stable for 18 months if stored properly.
Protocol
Available protocols: CUT&Tag
Specificity / Sensitivity
This kit can generate DNA libraries using CUT&Tag DNA. Note: While CUT&Tag DNA libraries generated for histone modifications typically show robust signal in Agilent Bioanalyzer or TapeStation systems analysis, libraries generated for non-histone proteins such as transcription factors and cofactors often have very weak or even no visible signal using Bioanalyzer or TapeStation systems, but still generate NG-sequencing results with high mapping rates, high numbers of identified binding peaks, and decent signal-to-noise ratios across the whole genome.
Species Reactivity: All Species Expected
Background
Similar to Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag) is a powerful technique used for probing protein-DNA interactions within the natural chromatin context of the cell (1-3). CUT&Tag has many of the same advantages as the CUT&RUN assay in that it provides a rapid, robust, and true low cell number protocol for detection of protein-DNA interactions in the cell. In addition, the CUT&Tag assay adds an adaptor DNA ligation step carried out by the pAG-Tn5 enzyme, in which an adaptor DNA is ligated directly to antibody-targeted chromatin DNA fragments in the cell. As a result, subsequent DNA library preparation is much faster and easier than library preparation following the CUT&RUN assay, free from DNA end repair, A-tailing, and adaptor ligation . CUT&Tag works very well for analyzing histone modifications, in addition to mapping some transcription factor and cofactor binding.
Alternate Names
Barcode; C&T; cut and tag; cut&tag; CUT&Tag; cutandtag; DNA library; DNA library construction; DNA library preparation; Index; next gen sequencing; NG-sequencing; NGS; Oligo; sequencing
