Description
Product Usage Information
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody detects endogenous levels of CBP or p300 only when acetylated at lysine 1535 or lysine 1499, respectively.
Species Reactivity: Human, Mouse, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys1535 of human CBP. Antibodies are purified by protein A and peptide affinity chromatography.
Background
CBP (CREB-binding protein) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). Phosphorylation of p300 at Ser89 by PKC represses its transcriptional activity, and phosphorylation at the same site by AMPK disrupts the association of p300 with nuclear receptors (3,4). Ser1834 phosphorylation of p300 by Akt disrupts its association with C/EBPβ (5). Growth factors induce phosphorylation of CBP at Ser437, which is required for CBP recruitment to the transcription complex (6). CaM kinase IV phosphorylates CBP at Ser302, which is required for CBP-dependent transcriptional activation in the CNS (7). The role of acetylation of CBP/p300 is of particular interest (2,8). Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (9).
Alternate Names
CBP; CREB binding protein; CREB-binding protein; CREBBP; E1A binding protein p300; E1A-associated protein p300; E1A-binding protein, 300kD; EP300; Histone acetyltransferase p300; Histone butyryltransferase p300; Histone crotonyltransferase p300; Histone lysine acetyltransferase CREBBP; KAT3A; KAT3B; MKHK1; MKHK2; P300; p300 HAT; Protein 2-hydroxyisobutyryltransferase p300; Protein propionyltransferase p300; Protein-lysine acetyltransferase CREBBP; RSTS; RSTS1; RSTS2
