Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Immunoprecipitation: 1:100
Immunofluorescence (Frozen): 1:100
Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Anillin (F6M2V) Rabbit Monoclonal Antibody recognizes endogenous levels of total anillin protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala14 of human anillin protein.
Background
Anillin/ANLN is an actin-binding protein with roles in cytokinesis and cell migration (1). Anillin is highly expressed in human cancer and is a potential prognostic biomarker and therapeutic target (2). In hepatocellular carcinoma (HCC), anillin expression is correlated with poor prognosis, and anillin deficiency induces multinucleated cells, apoptosis, and DNA damage checkpoints (3). Anillin knockdown in HCC leads to cell growth inhibition through impairment of cytokinesis (4). Anillin expression is negatively regulated by DNA damage in a p53-dependent manner (5).
Alternate Names
Actin-binding protein anillin; Anillin; anillin actin binding protein; anillin, actin binding protein; ANLN; DKFZp779A055; FSGS8; scra; Scraps
