Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:50 - 1:250
Immunofluorescence (Immunocytochemistry): 1:800 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
AKAP12 (F4H6G) Rabbit Monoclonal Antibody recognizes endogenous levels of total AKAP12 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect all isoforms of human AKAP12 protein. Reactivity for immunofluorescence is human only.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys407 of human AKAP12 protein.
Background
A-kinase anchoring protein 12 (AKAP12), also known as Gravin, is a scaffold protein that regulates intracellular signaling by sequestering signaling molecules in subcellular compartments as protein complexes. AKAP12 regulates protein kinase A (PKA) and protein kinase C (PKC)-dependent signaling and controls cell cycle progression, cytoskeletal dynamics, vascular development, glial function, and angiogenesis. AKAP12 expression is correlated with chronic liver diseases (CLDs), inflammatory diseases, and several human cancers (1,2). AKAP12 is a potential therapeutic target for the treatment of esophageal squamous cell carcinoma (ESCC) and colorectal cancer (3,4), and may serve as a biomarker for anti-VEGF therapy-resistant cancer, where its expression is upregulated (5). AKAP12 is highly expressed in heart, regulates desensitization and recycling of β2-adrenergic-receptor (β2AR), and has been implicated in cardiovascular disease initiation and progression (6). Increased expression of AKAP12 in heart is associated with accelerated cardiac dysfunction (7).
Alternate Names
A kinase (PRKA) anchor protein 12; A-kinase anchor protein 12; A-kinase anchor protein 250 kDa; A-kinase anchor protein, 250kDa; A-kinase anchoring protein 12; AKA12; AKAP 250; AKAP-12; AKAP12; AKAP250; DKFZp686M0430; DKFZp686O0331; FLJ20945; FLJ97621; Gravin; kinase scaffold protein gravin; Myasthenia gravis autoantigen; myasthenia gravis autoantigen gravin; Src-Suppressed C Kinase Substrate; SSeCKS
