CST, 49118C, FastScan™ Phospho-4E-BP1 (Thr37/46) ELISA Kit

Protocol
Available protocols: ELISA+
Specificity / Sensitivity
The FastScan™ Phospho-4E-BP1 (Thr37/46) ELISA Kit detects endogenous levels of 4E-BP1 when phosphorylated at Thr37/46, as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity: Human
Background
Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).
Alternate Names
4E-BP1; 4EBP1; BP-1; eIF4E-binding protein 1; EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; MGC4316; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1