Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
AID (EK2 5G9) Rat Monoclonal Antibody detects endogenous levels of total AID protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Asp191 of human AID.
Background
Activation-induced cytidine deaminase (AID) is thought to modify RNA due to its high homology to the RNA editing enzyme APOBEC-1. This function, however, has not been confirmed in studies, which show that AID has significant cytidine deaminase activity, and that this activity is blocked by zinc chelation (1). The B cell immune system must specifically recognize several infectious agents, which vastly outnumber immunoglobulin gene segments present in a given organism. Mechanisms such as somatic hypermutation, isotype switch recombination and gene conversion introduce diversity and specificity to the immune system. Analysis of mouse models and patients with AID deficiency has established a link between all three of these mechanisms and AID function (2). AID protein is detected in germinal center centroblast and germinal center derived lymphomas (Burkitt lymphoma), but not in pre-germinal center B cells or post-germinal center neoplasms (B cell chronic lymphocytic leukemia and multiple myeloma) (3).
Alternate Names
activation induced cytidine deaminase; Activation-induced cytidine deaminase; AICDA; AID; ARP2; CDA2; Cytidine aminohydrolase; epididymis secretory protein Li 284; HEL-S-284; HIGM2; integrated into Burkitt's lymphoma cell line Ramos; Single-stranded DNA cytosine deaminase
