Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ALOX12 (E3O9P) Rabbit Monoclonal Antibody recognizes endogenous levels of total ALOX12 protein.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly608 of human ALOX12 protein.
Background
Lipoxygenases catalyze the oxidation of polyunsaturated fatty acids and have a variety of physiological functions as well as roles in disease (1). ALOX12 (arachidonic acid 12-lipoxygenase) is an important lipoxygenase that specifically converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid (12- HpETE), which is then reduced by glutathione peroxidase to 12-hydroxyeicosatetraenoic acid (12-HETE). ALOX12 plays a role in regulating platelet aggregation, cell migration, and proliferation and is involved in diseases, such as thrombosis, atherosclerosis, diabetes, neurodegeneration, inflammatory disease, and cancer (2). ALOX12 is highly expressed in nonalcoholic steatohepatitis (NASH) and can regulate disease progression by interacting with acetyl-CoA carboxylase 1 (ACC1) (3,4). ALOX12 is also upregulated during hepatic ischemia leading to inflammation and cell death (5). ALOX12 inhibition also abrogates p53-mediated ferroptosis and tumor suppression but does not affect other ferroptosis pathways such as that driven by GPX4 (6).
Alternate Names
12-LOX; 12(S)-lipoxygenase; 12LO; 12S-lipoxygenase; 12S-LOX; ALOX12; Arachidonate (12S)-lipoxygenase; Arachidonate (15S)-lipoxygenase; arachidonate 12-lipoxygenase; arachidonate 12-lipoxygenase, 12S type; Arachidonate 12-lipoxygenase, 12S-type; arachidonate 15-lipoxygenase,15S-type; Linoleate (13S)-lipoxygenase; linoleate 13S-lipoxygenase; Lipoxin synthase 12-LO; LOG12; LOX12; platelet 12-LOX; platelet-type 12-lipoxygenase; Platelet-type lipoxygenase 12; Polyunsaturated fatty acid lipoxygenase ALOX12
